Step 2 - Using another slide, back onto the drop and pull the slide along the glass.
Step 3 - Allow to dry
Step 4 - Cover the slide with a layer of alcohol then allow to dry. This fixes the the blood cells
Step 5 - Cover the slide with haematoxylin for two minutes
Step 6 - Rinse in water for one minute to remove excess haematoxylin. This is called differentiation
Step 7 - Wash in 50% alcohol for one minute
Step 8 - Cover with eosin for 30 secs.
Step 9 - Wash in 50% alcohol again
Step 11 - Place a drop of mountant on the slide and cover with a coverslip.
= Nucleic of white blood cells- purple
= Cytoplasm of white blood cells- pink