Sunday, 2 August 2015

How to make a blood slide

Step 1 - Place a drop of blood on the slide

Step 2 - Using another slide, back onto the drop and pull the slide along the glass.

Step 3 - Allow to dry

Step 4 - Cover the slide with a layer of alcohol then allow to dry. This fixes the the blood cells

Step 5 - Cover the slide with haematoxylin for two minutes

Step 6 - Rinse in water for one minute to remove excess haematoxylin. This is called differentiation

Step 7 - Wash in 50% alcohol for one minute

Step 8 - Cover with eosin for 30 secs.

Step 9 - Wash in 50% alcohol again

Step 10 - Leave to dry

Step 11 - Place a drop of mountant on the slide and cover with a coverslip.

 = Nucleic of white blood cells- purple
=  Cytoplasm of white blood cells- pink

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